Prospects for functional genomics in Schizosaccharomyces pombe

  title={Prospects for functional genomics in Schizosaccharomyces pombe},
  author={Per Sunnerhagen},
  journal={Current Genetics},
Abstract.Schizosaccharomyces pombe is well established as an experimental organism for basic research, with well developed technologies for molecular biology, genetics and cell biology. Its full genome sequence has recently been published. Here, the prerequisites for functional genomics studies in Sch. pombe are examined and compared with those of some established prominent functional genomics model organisms, especially Saccharomyces cerevisiae. It is argued that functional genomics studies in… Expand
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Fingerprinting fission yeast: polymorphic markers for molecular genetic analysis of Schizosaccharomyces pombe strains.
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Rearrangements of the transcriptional regulatory networks of metabolic pathways in fungi.
The evolution of transcriptional regulatory networks governing the metabolic machinery of sequenced ascomycetes species is discussed and it is shown that transcriptional rewiring is common in regulons controlling processes such as production of ribosome components and metabolism of carbohydrates and lipids. Expand
Proteomic Study for the Cellular Responses to Cd2+ in Schizosaccharomyces pombe Through Amino Acid-coded Mass Tagging and Liquid Chromatography Tandem Mass Spectrometry*S
  • W. Bae, X. Chen
  • Chemistry, Medicine
  • Molecular & Cellular Proteomics
  • 2004
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A parallel proteomic and metabolomic analysis of the hydrogen peroxide‐ and Sty1p‐dependent stress response in Schizosaccharomyces pombe
The complexity of the cellular response to oxidative stress and the dependency on the Sty1p stress‐activated protein kinase is revealed and the relationship between these protein changes and mRNA expression levels identified in a parallel whole genome study is shown. Expand
Genetic interaction networks: toward an understanding of heritability.
Despite relatively low conservation of individual genes and their pairwise interactions, the overall topology of genetic interaction networks and the connections between broad biological processes may be similar in most organisms, which should provide a fundamental basis for mapping and predicting genetic interaction Networks in humans. Expand
Stress‐induced changes in the Schizosaccharomyces pombe proteome using two‐dimensional difference gel electrophoresis, mass spectrometry and a novel integrated robotics platform
The identification of all significantly altered proteins provides an annotated peroxide stress‐related proteome that can be used as a base resource against which other stress‐induced proteomic changes can be compared. Expand


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Featured Organism: Schizosaccharomyces pombe, The Fission Yeast
  • J. Wixon
  • Biology, Medicine
  • Comparative and functional genomics
  • 2002
A look at this important yeast, whose genome has recently been completed, featuring comments from Valerie Wood, Jürg Bähler, Ramsay McFarlane, Susan Forsburg, Iain Hagan and Paul Nurse on the implications of having the complete sequence and future prospects for pombe genomics. Expand
The model unicellular eukaryote, Schizosaccharomyces pombe
The fission yeast Schizosaccharomyces pombe has long been a model organism for studies of eukaryotic cells, winning renown especially for studies of the cell cycle. Now that its genome has beenExpand
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Genomic Exploration of the Hemiascomycetous Yeasts: 4. The genome of Saccharomyces cerevisiae revisited
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PCR-mediated direct gene disruption in Schizosaccharomyces pombe.
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Insertional mutagenesis based on illegitimate recombination in Schizosaccharomyces pombe.
An efficient insertional mutagenesis system has been developed for Schizosaccharomyces pombe based on linear PCR-generated cassettes containing selectable markers. It depends upon illegitimateExpand
Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe. Isolation of multiple genes conferring resistance.
It is shown that BFA has identical effects in the fission yeast Schizosaccharomyces pombe which normally contains a Golgi complex of stacked cisternae similar to the Golgi complexes in animal cells, which indicates that the effects of BFA in fungi are very similar to those in mammalian cells. Expand
Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe
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