Prospective isolation of human MSC.

  title={Prospective isolation of human MSC.},
  author={Abhishek Harichandan and H H B{\"u}hring},
  journal={Best practice & research. Clinical haematology},
  volume={24 1},
Conventionally, mesenchymal/stromal stem cells (MSC) are functionally isolated from primary tissue based on their capacity to adhere to the plastic surface. This isolation procedure is hampered by the unpredictable influence of secreted molecules or interactions with co-cultured hematopoietic and other unrelated cells as well as by the arbitrarily selected removal time of non-adherent cells prior to expansion of MSC. Early removal of non-adherent cells may result in the elimination of a late… CONTINUE READING

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