Properties of two endoglucanases from a mutant strain Trichoderma sp. M7 with potential application in the paper industry

@article{Petrova2009PropertiesOT,
  title={Properties of two endoglucanases from a mutant strain Trichoderma sp. M7 with potential application in the paper industry},
  author={Svetla D Petrova and N. G. Bakalova and D. N. Kolev},
  journal={Applied Biochemistry and Microbiology},
  year={2009},
  volume={45},
  pages={150-155}
}
Two endoglucanases were purified to electrophoretic homogeneity from the culture filtrate of a mutant strain Trichoderma sp. M7. EG-III and EG-IV had Mr of 49.7 and 47.5 kDa, and estimated pi values of 3.7 and 6.35, respectively. The optimal pH and temperature values were determined to be pH 5.0 and 60°C for the first cellulase, whereas pH 5.2 and 50 °C were optimal for the other. Endoglucanases exhibited typical Michaelis-Menten kinetics with K m and V values of 2.9 mg ml−1 and 60498.5 μmol… CONTINUE READING