Properties of trypsin and of acid phosphatase immobilized in sol-gel glass matrices.

@article{Shtelzer1992PropertiesOT,
  title={Properties of trypsin and of acid phosphatase immobilized in sol-gel glass matrices.},
  author={S Shtelzer and Sara Rappoport and David Avnir and Michael Ottolenghi and Sergei Braun},
  journal={Biotechnology and applied biochemistry},
  year={1992},
  volume={15 3},
  pages={227-35}
}
Trypsin and acid phosphatase-containing silica sol-gel glasses were obtained by mixing a solution of an enzyme with polyethylene glycol (PEG) 6000 and tetramethoxy orthosilicate at room temperature, followed by gelation and drying. Activity of the immobilized trypsin toward small substrates, such as N-benzoyl-L-arginine-4-nitroanilide at its Km, for the… CONTINUE READING