Properties of the visual pigments of the moth Manduca sexta and the effects of two detergents, digitonin and chaps.

@article{Bennett1985PropertiesOT,
  title={Properties of the visual pigments of the moth Manduca sexta and the effects of two detergents, digitonin and chaps.},
  author={Ruth R. Bennett and Paul K. Brown},
  journal={Vision research},
  year={1985},
  volume={25 12},
  pages={1771-81}
}
The three known visual pigments (P520, P450, P357) of the moth, Manduca sexta (Lepidoptera, Sphingidae), were extracted in two different detergents (2% digitonin, 6 or 12 mM CHAPS). As is the case in unextracted membranes, the metarhodopsins are quite stable in CHAPS extracts, while in digitonin the metarhodopsins of P520 and P450 decay rapidly at 15 degrees C to opsin and free retinal. The relative absorbance ratios are: 1.0:1.6 (P520:M485), 1.0:1.1 (P450:M485), and 1.0:0.8 (P357:M470). The… CONTINUE READING

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