Properties of the two terminal oxidases of Escherichia coli.

  title={Properties of the two terminal oxidases of Escherichia coli.},
  author={Anne Puustinen and Moshe Finel and Tuomas Haltia and Robert B Gennis and M{\aa}rten Wikstr{\"o}m},
  volume={30 16},
Proton translocation coupled to oxidation of ubiquinol by O2 was studied in spheroplasts of two mutant strains of Escherichia coli, one of which expresses cytochrome d, but not cytochrome bo, and the other expressing only the latter. O2 pulse experiments revealed that cytochrome d catalyzes separation of the protons and electrons of ubiquinol oxidation but is not a proton pump. In contrast, cytochrome bo functions as a proton pump in addition to separating the charges of quinol oxidation. E… CONTINUE READING