Properties of the human Cdc45/Mcm2-7/GINS helicase complex and its action with DNA polymerase epsilon in rolling circle DNA synthesis.

@article{Kang2012PropertiesOT,
  title={Properties of the human Cdc45/Mcm2-7/GINS helicase complex and its action with DNA polymerase epsilon in rolling circle DNA synthesis.},
  author={Young-Hoon Kang and Wiebke Chemnitz Galal and Andrea Farina and Inger Tappin and Jerard Hurwitz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2012},
  volume={109 16},
  pages={6042-7}
}
In eukaryotes, although the Mcm2-7 complex is a key component of the replicative DNA helicase, its association with Cdc45 and GINS (the CMG complex) is required for the activation of the DNA helicase. Here, we show that the CMG complex is localized to chromatin in human cells and describe the biochemical properties of the human CMG complex purified from baculovirus-infected Sf9 cells. The isolated complex binds to ssDNA regions in the presence of magnesium and ATP (or a nonhydrolyzable ATP… CONTINUE READING

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