Properties of some nuclear nucleases of rat thymocytes and their changes in radiation-induced apoptosis.

@article{Nikonova1993PropertiesOS,
  title={Properties of some nuclear nucleases of rat thymocytes and their changes in radiation-induced apoptosis.},
  author={L V Nikonova and Igor P Beletsky and Samuil R. Umansky},
  journal={European journal of biochemistry},
  year={1993},
  volume={215 3},
  pages={893-901}
}
Three nuclease activities have been found and characterized in rat thymocyte nuclear extracts. A Mn(2+)-dependent nuclease is loosely bound to nuclear components and can be extracted with 0.35 M NaCl. The enzyme is activated by Mn2+ but not by Mg2+, Ca2+, or both. Its molecular mass is 36-40 kDa when measured by gel filtration and 37 kDa by SDS/PAGE. An acidic nuclease is independent of divalent ions, produces DNA strand breaks with 5'-OH ends, its molecular mass is about 37 kDa. Two fractions… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

Similar Papers

Loading similar papers…