Properties of plasma membrane-induced amylase release from rat parotid secretory granules: effects of Ca2+ and Mg-ATP.

@article{Mizuno1992PropertiesOP,
  title={Properties of plasma membrane-induced amylase release from rat parotid secretory granules: effects of Ca2+ and Mg-ATP.},
  author={Morimichi Mizuno and Yasunaga Kameyama and Koji Yashiro and Sun Oh Shin and Yukako Yokota},
  journal={Biochimica et biophysica acta},
  year={1992},
  volume={1116 2},
  pages={104-11}
}
A secretory granular fraction (SG) and a plasma membrane rich fraction (PM) have been isolated from rat parotid gland by differential and Percoll gradient centrifugation. With these two fractions, a cell-free interaction system has been reconstituted to clarify the exocytotic interaction between the secretory granules and plasma membranes, and the conditions of amylase release from SG have been characterized in vitro. The addition of PM into this assay system induced a rapid and transient… CONTINUE READING