Properties of neurons from dissociated fetal rat brain in serum-free culture.

  title={Properties of neurons from dissociated fetal rat brain in serum-free culture.},
  author={Zahur Ahmed and P S Walker and Robert E. Fellows},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  volume={3 12},
Because of the unknown constituents and varying composition of serum, its presence in media used in cell culture unavoidably compromises attempts to study cellular mechanisms of growth and differentiation. To overcome this, we have devised a serum-free, chemically defined medium which maintains primary cultures of fetal rat brain cells for more than 6 weeks. This medium allows expression of characteristic properties of neurons and prevents overgrowth of non-neuronal elements without use of… CONTINUE READING
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