Properties of liver retinyl ester hydrolase in young pigs.

  title={Properties of liver retinyl ester hydrolase in young pigs.},
  author={Dr. Arthur J. L. Cooper and James A. Olson},
  journal={Biochimica et biophysica acta},
  volume={884 2},
A new sensitive method for the assay of retinyl ester hydrolase in vitro was developed and applied to liver homogenates of 18 young pigs with depleted-to-adequate liver vitamin A reserves. Radioactive substrate was not required, because the formation of retinol could be adequately quantitated by reversed-phase high-performance liquid chromatography. Optimal hydrolase activity was observed with 500 microM retinyl palmitate, 100 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, and 2… CONTINUE READING