Properties of a Lactobacillus fermenti bacteriocin.

  title={Properties of a Lactobacillus fermenti bacteriocin.},
  author={Hennie de Klerk and Jacobus A. Smit},
  journal={Journal of general microbiology},
  volume={48 2},
SUMMARY: The properties of a bacteriocin derived from Lactobacillus fermenti strain 466 were investigated. The bacteriocin was present in low titre in supernatant fluids from overnight broth cultures and was not inducible by ultraviolet radiation. It was purified and concentrated to a titre of 1/1000 by dialysis, chromatography on Sephadex G100 and calcium phosphate gel columns. The bacteriocin is heat stable, and sensitive to trypsin and pepsin but not to lysozyme. No migration was… 

Bacteriocin of a Group A Streptococcus: Partial Purification and Properties

The production of bacteriocin-like inhibition by certain strains of group A streptococci was demonstrated during the growth of these organisms on solid nutrient media and, although labile in alkali, it was extremely stable to heating in mild acids.

Production and Purification of a Staphylococcus epidermidis Bacteriocin

Liquid cultures of Staphylococcus epidermidis 1580 contained rather small amounts of a bacteriocin, staphylococcin 1580, which was found both in the supernatant fluid and in the cell pellet. It could

Isolation and Characterization of a Bacteriocin from a Homofermentative Lactobacillus

Nearly 100 isolates of Lactobacillus were obtained from human and animal sources and one of these, L. helveticus strain LP27, was shown to produce a potent bacteriocin called lactocin 27, which was isolated from the culture supernatant fluid as a protein-lipopolysaccharide complex.

Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B.

Characteristics identified for lactacin B indicated that it was a peptide and confirmed its identity as a bacteriocin and its activity was bactericidal to sensitive cells and restricted to members of the family Lactobacilliaceae.

Caseicin 80: purification and characterization of a new bacteriocin from Lactobacillus casei

The bacteriocin which the authors called caseicin 80, was also detectable in cell extracts, although only 2% of the total activity was retained intracellularly and the isoelectric point was pH 4.5.

Purification andCharacterization oftheLactobacillus acidophilus Bacteriocin Lactacin Bt

Characteristics identified for lactacin B indicated that it was a peptide and confirmed its identity as a bacteriocin, which is antagonistic proteins or peptides that show bactericidal activity against closely related species.

Production and Mode of Action of Lactocin 27: Bacteriocin from a Homofermentative Lactobacillus

Lactobacillus helveticus strain LP27 produced a bacteriocin, lactocin 27, in dialyzable and nondialyzable forms, which inhibited primarily protein synthesis without affecting deoxyribonucleic acid and ribon nucleic acid synthesis or adenosine 5′-triphosphate levels.

Isolation of a Lactobacillus salivarius Strain and Purification of Its Bacteriocin, Which Is Inhibitory to Campylobacter jejuni in the Chicken Gastrointestinal System

Bacteriocin from L. salivarius NRRL B-30514 appears potentially very useful to reduce C. jejuni in poultry prior to processing, and had sequence similarity, even in the C-terminal region, to acidocin A, which was previously identified fromL.

Characterization and isolation of a bacteriocin produced by a strain of Propionibacterium thoenii

A partially purified bacteriocin produced by Propionibacterium thoenii designated propionicin PLG-1 was found to be active against closely related species and exhibited a broad spectrum of activity

Bacteriocinogeny in Lactobacillus fermenti

BACTERIOCINS are antibiotics produced by bacteria and which act on strains of the same or closely related species. They are protein in nature. De Klerk and Coetzee1 reported bacteriocin production by


Two strains of Streptococcus zymogenes produce a bacteriocine which is active against lactic acid bacteria and most other grampositive bacteria, and both activities are stable when heated in agar.

The purification and properties of megacin, a bacteriocin from Bacillus megaterium.

Ivanovics, Alfoldi & Nagy (1959), using partially purified preparations, obtained some evidence that megacin acts on the cytoplasmic membrane of sensitive cells, and isolate a highly purified preparation ofmegacin to investigate its physicochemical and ultimately its biological properties.

Cell wall composition of lactic acid bacteria.


SUMMARY: An improved growth medium for lactobacilli is described. It supports good growth of lactobacilli generally and also is particularly useful for a number of fastidious strains which grow only

Protein chromatography on calcium phosphate columns.

Method for the determination of hexosamines in tissues.

  • N. F. Boas
  • Biology
    The Journal of biological chemistry
  • 1953

The amino-aciduria in Fanconi syndrome. A study making extensive use of techniques based on paper partition chromatography.

  • C. Dent
  • Medicine
    The Biochemical journal
  • 1947
The introduction by Consden, Gordon & Martin (1944) of a comparatively simple technique for the detection of amino-acids has provided a most effective weapon for the purpose and has already been shown to be applicable to pathological specimens of urine and other body fluids.

A study of the negative staining process.

  • D. E. Bradley
  • Materials Science
    Journal of general microbiology
  • 1962
The effectiveness of a number of different materials in the negative staning method for electron microscopy of viruses is evaluated: perforated carbon films were found valuable for obtaining good contrast and it was important to de-grease supporting film.