Properties of a Clostridium thermocellum Endoglucanase Produced in Escherichia coli.

  title={Properties of a Clostridium thermocellum Endoglucanase Produced in Escherichia coli.},
  author={Wolfgang H Schwarz and Folke Graebnitz and W. L. Staudenbauer},
  journal={Applied and environmental microbiology},
  volume={51 6},
A cellulase gene of Clostridium thermocellum was transferred to Escherichia coli by molecular cloning with bacteriophage lambda and plasmid vectors and shown to be indentical with the celA gene. The celA gene product was purified from extracts of plasmid-bearing E. coli cells by heat treatment and chromatography on DEAE-Trisacryl. It was characterized as a thermophilic endo-beta-1,4-glucanase, the properties of which closely resemble those of endoglucanase A previously isolated from C… CONTINUE READING
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