Properties of Trypsin from the Pyloric Ceca of Atlantic Cod (Gadus morhua)

@article{Simpson1990PropertiesOT,
  title={Properties of Trypsin from the Pyloric Ceca of Atlantic Cod (Gadus morhua)},
  author={B. Simpson and M. V. Simpson and N. Haard},
  journal={Journal of Food Science},
  year={1990},
  volume={55},
  pages={959-961}
}
Trypsin (EC 3.4.21.4) was isolated from the pyloric ceca of Atlantic cod and purified to homogeneity by affinity chromatography. The enzyme catalyzed the hydrolysis of benzoyl arginine p-nitroanilide (BAPA, pH 8.2 and 25°C) such that Vmax was 250 BAPA units per micromole trypsin and Km was 1.48 mM. For the hydrolysis of tosyl arginine methyl ester (TAME, pH 8.1 and 25°C), Vmax was 18.2 × 103 TAME units/micromole trypsin, and Km 0.22 mM. The pH and temperature optima with BAPA substrate were 7.5… Expand
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