Properties of Single Voltage-gated Proton Channels in Human Eosinophils Estimated by Noise Analysis and by Direct Measurement

@article{Cherny2003PropertiesOS,
  title={Properties of Single Voltage-gated Proton Channels in Human Eosinophils Estimated by Noise Analysis and by Direct Measurement},
  author={Vladimir Cherny and Ricardo Murphy and V S Sokolov and Richard A. Levis and Thomas E DeCoursey},
  journal={The Journal of General Physiology},
  year={2003},
  volume={121},
  pages={615 - 628}
}
Voltage-gated proton channels were studied under voltage clamp in excised, inside-out patches of human eosinophils, at various pHi with pHo 7.5 or 6.5 pipette solutions. H+ current fluctuations were observed consistently when the membrane was depolarized to voltages that activated H+ current. At pHi < or = 5.5 the variance increased nonmonotonically with depolarization to a maximum near the midpoint of the H+ conductance-voltage relationship, gH-V, and then decreased, supporting the idea that… CONTINUE READING