Properties of H+-translocating adenosine triphosphatase in vacuolar membranes of SAccharomyces cerevisiae.

@article{Kakinuma1981PropertiesOH,
  title={Properties of H+-translocating adenosine triphosphatase in vacuolar membranes of SAccharomyces cerevisiae.},
  author={Yoshimi Kakinuma and Yoshinori Ohsumi and Yasuhiro Anraku},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 21},
  pages={10859-63}
}
The properties of Mg2+-ATPase in the vacuole of Saccharomyces cerevisiae were studied, using purified intact vacuoles and right-side-out vacuolar membrane vesicles prepared by the method of Y. Ohsumi and Y. Anraku ((1981) J. Biol. Chem. 256, 2079). The enzyme requires Mg2+ ion but not Ca2+ in. Cu2+ and Zn2+ ions inhibit the activity. The optimal pH is at pH 7.0. The enzyme hydrolyzes ATP, GTP, UTP, and CTP in this order and the Km value for ATP was determined as 0.2 mM. It does not hydrolyze… CONTINUE READING

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