Properties of Chitosanase from Bacillus cereus S1

  title={Properties of Chitosanase from Bacillus cereus S1},
  author={Masahiro Kurakake and Shou Yo-u and Kiyomi Nakagawa and Minako Sugihara and Toshiaki Komaki},
  journal={Current Microbiology},
Chitosanase from Bacillus cereus S1 was purified, and the enzymatic properties were investigated. The molecular weight was estimated to 45,000 on SDS-PAGE. Optimum pH was about 6, and stable pH in the incubation at 40°C for 60 min was 6–11. This chitosanase was stable in alkaline side. Optimum temperature was around 60°C, and enzyme activity was relatively stable below 60°C. The degradations of colloidal chitosan and carboxymethyl cellulose (CMC) were about 30 and 20% relative to the value of… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 18 extracted citations


Publications referenced by this paper.
Showing 1-10 of 10 references

Purification and characterization of an extracellular chitosanase produced by Amycolatopsissp. CsO-2

  • S Okajima, A Ando, H Shinoyama, T Fujii
  • J Ferment Bioeng
  • 1994
Highly Influential
5 Excerpts

Properties and action pattern of a chitosanase from Bacillussp. PI-7S

  • H Seino, K Tsukada, Y Shimasue
  • Agric Biol Chem
  • 1991
Highly Influential
7 Excerpts

Purification and properties of chitosanase from Bacillus circulans MH-K1. L

  • M Yabuki, A Uchiyama, K Suzuki, A Ando, T Fujii
  • Gen Appl Microbiol
  • 1988
Highly Influential
6 Excerpts

Purification and characterization of chitosanases produced by Bacillus licheniformisUTK

  • Y Uchida, K Tateishi, K Yamaguchi, H Takeda
  • Bull Fac Agr Saga
  • 1994

Purification and properties of two endochitosanases from Mucor rouxii implicated in its cell wall degradation

  • C Alfonos, MJ Martines, F Reyes
  • FEMS Microbiol Lett
  • 1992

Preparation of D-glucosamine oligosaccharides by the enzymatic hydrolysis of chitosan

  • M Izume, A Ohtakara
  • Agric Biol Chem
  • 1987

Use of dinitrosalicilic acid reagent for determination of reducing sugar

  • GL Miller
  • Anal Chem
  • 1959
1 Excerpt

Similar Papers

Loading similar papers…