Properties of Chitosanase from Bacillus cereus S1

@article{Kurakake2000PropertiesOC,
  title={Properties of Chitosanase from Bacillus cereus S1},
  author={Masahiro Kurakake and Shou Yo-u and Kiyomi Nakagawa and Minako Sugihara and Toshiaki Komaki},
  journal={Current Microbiology},
  year={2000},
  volume={40},
  pages={6-9}
}
Chitosanase from Bacillus cereus S1 was purified, and the enzymatic properties were investigated. The molecular weight was estimated to 45,000 on SDS-PAGE. Optimum pH was about 6, and stable pH in the incubation at 40°C for 60 min was 6–11. This chitosanase was stable in alkaline side. Optimum temperature was around 60°C, and enzyme activity was relatively stable below 60°C. The degradations of colloidal chitosan and carboxymethyl cellulose (CMC) were about 30 and 20% relative to the value of… CONTINUE READING

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