Properties of Bacillus subtilis ATP-dependent deoxyribonuclease.

@article{Shemyakin1979PropertiesOB,
  title={Properties of Bacillus subtilis ATP-dependent deoxyribonuclease.},
  author={M. F. Shemyakin and A A Grepachevsky and A V Chestukhin},
  journal={European journal of biochemistry},
  year={1979},
  volume={98 2},
  pages={417-23}
}
A purification procedure described previously resulting in electrophoretically pure Bacillus subtilis ATP-dependent DNAse has now been modified by adding a fractionation stage with Polymin P to permit large-scale isolation of the enzyme. It has been found that the enzyme molecule (Mr = 300000) consists of two large subunits with Mr 155000 and 140000. The purified enzyme has three activities: (1) DNAse on linear single-stranded and double-stranded DNAs (2) DNA-unwinding and (3) ATPase. Circular… CONTINUE READING