Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum.

@article{Macis1998PropertiesAS,
  title={Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum.},
  author={Luciana Macis and Rolf Daniel and Gerhard Gottschalk},
  journal={FEMS microbiology letters},
  year={1998},
  volume={164 1},
  pages={
          21-8
        }
}
The genes encoding coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum were subcloned and expressed in Escherichia coli. The native molecular mass of the enzyme is 190,000 Da. The enzyme converts glycerol, 1,2-propanediol and 1,2-ethanediol to 3-hydroxypropionaldehyde, propionaldehyde and acetaldehyde, respectively, but glycerol is the preferred substrate. The nucleotide sequences of the dhaBCE genes encoding the three subunits of glycerol dehydratase and of orfZ whose… CONTINUE READING

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