Properties and applications of starch-converting enzymes of the α-amylase family

@article{Maarel2002PropertiesAA,
  title={Properties and applications of starch-converting enzymes of the $\alpha$-amylase family},
  author={Marc van der Maarel and Bart A. van der Veen and Joost C. M. Uitdehaag and Hans Leemhuis and Lubbert Dijkhuizen},
  journal={Journal of Biotechnology},
  year={2002},
  volume={94},
  pages={137-155}
}
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142 References

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Advances in microbial amylases.

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Commodity scale production of sugars from starches.

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