Proper fatty acid composition rather than an ionizable lipid amine is required for full transport function of lactose permease from Escherichia coli.

@article{Vitrac2013ProperFA,
  title={Proper fatty acid composition rather than an ionizable lipid amine is required for full transport function of lactose permease from Escherichia coli.},
  author={Heidi Vitrac and Mikhail Bogdanov and William Dowhan},
  journal={The Journal of biological chemistry},
  year={2013},
  volume={288 8},
  pages={5873-85}
}
Energy-dependent uphill transport but not energy-independent downhill transport by lactose permease (LacY) is impaired when expressed in Escherichia coli cells or reconstituted in liposomes lacking phosphatidylethanolamine (PE) and containing only anionic phospholipids. The absence of PE results in inversion of the N-terminal half and misfolding of periplasmic domain P7, which are required for uphill transport of substrates. Replacement of PE in vitro by lipids with no net charge… CONTINUE READING

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