Propagation of plant viruses in hairy root cultures: a potential method for in vitro production of epitope vaccines and foreign proteins.

@article{Shadwick2007PropagationOP,
  title={Propagation of plant viruses in hairy root cultures: a potential method for in vitro production of epitope vaccines and foreign proteins.},
  author={Fiona S Shadwick and Pauline M Doran},
  journal={Biotechnology and bioengineering},
  year={2007},
  volume={96 3},
  pages={570-83}
}
Hairy roots were used as an in vitro culture system for the propagation of wild-type and transgenic plant viruses. Tobacco mosaic virus (TMV) was added to the liquid culture medium at the same time as root inoculation. Hairy root growth was unaffected by viral infection. Maximum concentrations of TMV in Nicotiana benthamiana hairy roots were 1-2 orders of magnitude greater than in suspended N. benthamiana cells and reached levels of 1-2 mg g(-1) dry weight or 20-28% total soluble protein. Virus… CONTINUE READING

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