Propagation of human spermatogonial stem cells in vitro.

@article{SadriArdekani2009PropagationOH,
  title={Propagation of human spermatogonial stem cells in vitro.},
  author={Hooman Sadri-Ardekani and Sefika Canan Mizrak and Saskia K. M. van Daalen and Cindy M. Korver and Hermien L Roepers-Gajadien and Morteza Koruji and Suzanne E Hovingh and T M de Reijke and Jean J. M. C. H. de la Rosette and F. J. C. van der Veen and Dirk G de Rooij and Sjoerd Repping and Ans M.M. van Pelt},
  journal={JAMA},
  year={2009},
  volume={302 19},
  pages={2127-34}
}
CONTEXT Young boys treated with high-dose chemotherapy are often confronted with infertility once they reach adulthood. Cryopreserving testicular tissue before chemotherapy and autotransplantation of spermatogonial stem cells at a later stage could theoretically allow for restoration of fertility. OBJECTIVE To establish in vitro propagation of human spermatogonial stem cells from small testicular biopsies to obtain an adequate number of cells for successful transplantation. DESIGN, SETTING… CONTINUE READING

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