Propagation of Aleutian disease parvovirus in cell line CCC clone 81

@article{Dawen1983PropagationOA,
  title={Propagation of Aleutian disease parvovirus in cell line CCC clone 81},
  author={Sabine van Dawen and O. -R. Kaaden and Susanne Roth},
  journal={Archives of Virology},
  year={1983},
  volume={77},
  pages={39-50}
}
Aleutian disease parvovirus (ADV), mutant Gorham of the Utah-1 strain, was grown and comparatively assayed in feline cell lines CRFK and CCC clone 81 at 31.8°C. The maximum virus titres as determined by a fluorescent focus assay were found to be about 105 FFU/ml in CRFK at day 6 p. i. and 106 FFU/ml at day 4 p. i. in CCC clone 81 cells. Shifting of the incubation temperature from 31.8 to 37°C led to a reduced virus production after three passages. The synchronization of the CCC clone 81 cells… CONTINUE READING
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