Propagating the missing bacteriophages: a large bacteriophage in a new class

@article{Serwer2007PropagatingTM,
  title={Propagating the missing bacteriophages: a large bacteriophage in a new class},
  author={Philip Serwer and Shirley J. Hayes and Julie A. Thomas and S. C. Hardies},
  journal={Virology Journal},
  year={2007},
  volume={4},
  pages={21 - 21}
}
The number of successful propagations/isolations of soil-borne bacteriophages is small in comparison to the number of bacteriophages observed by microscopy (great plaque count anomaly). As one resolution of the great plaque count anomaly, we use propagation in ultra-dilute agarose gels to isolate a Bacillus thuringiensis bacteriophage with a large head (95 nm in diameter), tail (486 × 26 nm), corkscrew-like tail fibers (187 × 10 nm) and genome (221 Kb) that cannot be detected by the usual… CONTINUE READING

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