Proofreading genotyping assays mediated by high fidelity exo+ DNA polymerases.


DNA polymerases with 3'-5' proofreading function mediate high fidelity DNA replication but their application for mutation detection was almost completely neglected before 1998. The obstacle facing the use of exo(+) polymerases for mutation detection could be overcome by primer-3'-termini modification, which has been tested using allele-specific primers with 3' labeling, 3' exonuclease-resistance and 3' dehydroxylation modifications. Accordingly, three new types of single nucleotide polymorphism (SNP) assays have been developed to carry out genome-wide genotyping making use of the fidelity advantage of exo(+) polymerases. Such SNP assays might also provide a novel approach for re-sequencing and de novo sequencing. These new mutation detection assays are widely adaptable to a variety of platforms, including real-time PCR, multi-well plate and microarray technologies. Application of exo(+) polymerases to genetic analysis could accelerate the pace of personalized medicine.

Cite this paper

@article{Zhang2005ProofreadingGA, title={Proofreading genotyping assays mediated by high fidelity exo+ DNA polymerases.}, author={Jia Zhang and Kai Li and Jose R. Pardinas and Steve S. Sommer and Kai-Tai Yao}, journal={Trends in biotechnology}, year={2005}, volume={23 2}, pages={92-6} }