Proof-of-principle for SERS imaging of Aspergillus nidulans hyphae using in vivo synthesis of gold nanoparticles.
@article{Prusinkiewicz2012ProofofprincipleFS, title={Proof-of-principle for SERS imaging of Aspergillus nidulans hyphae using in vivo synthesis of gold nanoparticles.}, author={Martin A. Prusinkiewicz and Fatemeh Farazkhorasani and James J. Dynes and Jian Wang and Kathleen M. Gough and Susan G W Kaminskyj}, journal={The Analyst}, year={2012}, volume={137 21}, pages={ 4934-42 } }
High spatial resolution methods to assess the physiology of growing cells should permit analysis of fungal biochemical composition. Whole colony methods cannot capture the details of physiology and organism-environment interaction, in part because the structure, function and composition of fungal hyphae vary within individual cells depending on their distance from the growing apex. Surface Enhanced Raman Scattering (SERS) can provide chemical information on materials that are in close contact…
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References
SHOWING 1-10 OF 48 REFERENCES
Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: a mechanism perspective.
- BiologyNanoscale
- 2011
The present study demonstrates an eco-friendly and low cost protocol for synthesis of silver nanoparticles using the cell-free filtrate of Aspergillus flavus NJP08 when supplied with aqueous silver…
Extracellular Biosynthesis of Gold Nanoparticles using Aspergillus niger – its Characterization and Stability
- Biology, Chemistry
- 2009
Different characterization studies showed that the extracellular enzyme secreted by Aspergillus niger NCIM 616 might be responsible for both formation and capping of the metal nanoparticles.
Biosynthesis of silver, gold and bimetallic nanoparticles using the filamentous fungus Neurospora crassa.
- Materials ScienceColloids and surfaces. B, Biointerfaces
- 2011
Microbial synthesis of multishaped gold nanostructures.
- Materials ScienceSmall
- 2010
Electron diffraction patterns reveal the crystalline nature of the nanoparticles and a probable mechanism is proposed for the formation of the different structural entities.
Imaging the cell wall of living single yeast cells using surface-enhanced Raman spectroscopy
- Chemistry, BiologyAnalytical and bioanalytical chemistry
- 2009
The surface of a living yeast cell has been labeled with silver (Ag) nanoparticles that can form nanoaggregates which have been shown to have surface-enhanced Raman scattering (SERS) activity and the SERS spectra measured from different Ag nanoaggRegates were found to be different.
A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions
- BiologyAnalytical and bioanalytical chemistry
- 2007
The first results of the synchrotron FTIR examination of hyphae of three important fungal model systems, each with sequenced genomes and a wealth of research are reported, with significant differences between the spectra of the three fungi.
A sensitive method for examining whole-cell biochemical composition in single cells of filamentous fungi using synchrotron FTIR spectromicroscopy.
- BiologyJournal of inorganic biochemistry
- 2008
Time fluctuations and imaging in the SERS spectra of fungal hypha grown on nanostructured substrates.
- Chemistry, PhysicsThe journal of physical chemistry. B
- 2007
Time fluctuations in the intensity and band position in the SERS spectra measured on the same sample position with 1 s integration time have been observed indicating that the Sers signal arises from a limited number of molecules and that possibly single components are being detected.
High spatial resolution analysis of fungal cell biochemistry--bridging the analytical gap using synchrotron FTIR spectromicroscopy.
- BiologyFEMS microbiology letters
- 2008
Fungi impact humans and the environment in many ways, for good and ill. Some fungi support the growth of terrestrial plants or are used in biotechnology, and yet others are established or emerging…
Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus
- Materials Science, Chemistry
- 2007