Proof-of-principle for SERS imaging of Aspergillus nidulans hyphae using in vivo synthesis of gold nanoparticles.

  title={Proof-of-principle for SERS imaging of Aspergillus nidulans hyphae using in vivo synthesis of gold nanoparticles.},
  author={Martin A. Prusinkiewicz and Fatemeh Farazkhorasani and James J. Dynes and Jian Wang and Kathleen M. Gough and Susan G W Kaminskyj},
  journal={The Analyst},
  volume={137 21},
High spatial resolution methods to assess the physiology of growing cells should permit analysis of fungal biochemical composition. Whole colony methods cannot capture the details of physiology and organism-environment interaction, in part because the structure, function and composition of fungal hyphae vary within individual cells depending on their distance from the growing apex. Surface Enhanced Raman Scattering (SERS) can provide chemical information on materials that are in close contact… 

Figures and Tables from this paper

Properties of in situ generated gold nanoparticles in the cellular context.

The biomolecular corona of nanoparticles formed in situ after incubation in buffer and DMEM differs, suggesting that different intracellular molecular species serve for reduction and stabilization.

Surface-Enhanced Raman Spectroscopy (SERS) Cellular Imaging of Intracellulary Biosynthesized Gold Nanoparticles

SERS imaging was used to detect intracellular and extracellular gold nanoparticles biosynthesized by the green algae Pseudokirchneriella subcapitata to identify surface-associated biomolecules and to evaluate the nanoparticle biosynthesis mechanism.

SERS Properties of Different Sized and Shaped Gold Nanoparticles Biosynthesized under Different Environmental Conditions by Neurospora crassa Extract

Gold nanoparticles with good SERS properties are synthesized by Neurospora crassa extract under different environmental conditions, increasing Raman signals up to 40 times using methylene blue as a target molecule, showing that these ‘green’ synthesized gold nanoparticles might have potential applicability in the field of biological sensing.

Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose.

This work explores gene deletion strains lacking the penultimate enzyme in Galf biosynthesis and the protein that transports Galf for incorporation into the cell wall (ugtAΔ) and demonstrates correlative data collection with these two emerging modalities for the multiplexed in situ study of the nanoscale architecture and chemical composition of fungal cell walls.

Silver nanoparticles: a mechanism of action on moulds.

The ultrastructure analysis revealed many sever alterations due to the action of AgNPs, such us shortening and condensation of hyphae, ultrastructural reorganisation, cell plasmolysis, increased vacuolisation, numerous membranous structures, collapsed cytoplasm and accumulation of lipid material.

Spectroscopic analysis of mushrooms by surface-enhanced Raman scattering (SERS)

Background Mushrooms have always been considered an important source of food and biologically active compounds with several medicinal properties. In recent years, different methods were used to study

Fungal Biosynthesis of Nanoparticles, a Cleaner Alternative

In this chapter, the state of the art in fungal biosynthesis of metallic NPs is reviewed and the use of nonpathogenic fungi will greatly improve the biocompatibility of the produced nanomaterial.

X-ray microfluorescence (μXRF) imaging of Aspergillus nidulans cell wall mutants reveals biochemical changes due to gene deletions

It is demonstrated that deleting a dispensable gene involved in galactose metabolism (ugeA) and one involved in biosynthesis of a minor cell wall component (ugmA) led to changes in hyphal elemental distribution that may have resulted from compromised wall composition.

Viability of Cladosporium herbarum spores under 157 nm laser and vacuum ultraviolet irradiation, low temperature (10 K) and vacuum

Ultraviolet photons can damage microorganisms, which rarely survive prolonged irradiation. In addition to the need for intact DNA, cell viability is directly linked to the functionality of the cell



Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: a mechanism perspective.

The present study demonstrates an eco-friendly and low cost protocol for synthesis of silver nanoparticles using the cell-free filtrate of Aspergillus flavus NJP08 when supplied with aqueous silver

Extracellular Biosynthesis of Gold Nanoparticles using Aspergillus niger – its Characterization and Stability

Different characterization studies showed that the extracellular enzyme secreted by Aspergillus niger NCIM 616 might be responsible for both formation and capping of the metal nanoparticles.

Microbial synthesis of multishaped gold nanostructures.

Electron diffraction patterns reveal the crystalline nature of the nanoparticles and a probable mechanism is proposed for the formation of the different structural entities.

Imaging the cell wall of living single yeast cells using surface-enhanced Raman spectroscopy

The surface of a living yeast cell has been labeled with silver (Ag) nanoparticles that can form nanoaggregates which have been shown to have surface-enhanced Raman scattering (SERS) activity and the SERS spectra measured from different Ag nanoaggRegates were found to be different.

A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions

The first results of the synchrotron FTIR examination of hyphae of three important fungal model systems, each with sequenced genomes and a wealth of research are reported, with significant differences between the spectra of the three fungi.

Time fluctuations and imaging in the SERS spectra of fungal hypha grown on nanostructured substrates.

Time fluctuations in the intensity and band position in the SERS spectra measured on the same sample position with 1 s integration time have been observed indicating that the Sers signal arises from a limited number of molecules and that possibly single components are being detected.

High spatial resolution analysis of fungal cell biochemistry--bridging the analytical gap using synchrotron FTIR spectromicroscopy.

Fungi impact humans and the environment in many ways, for good and ill. Some fungi support the growth of terrestrial plants or are used in biotechnology, and yet others are established or emerging