Proof of principle and first cases using preimplantation genetic haplotyping--a paradigm shift for embryo diagnosis.

@article{Renwick2006ProofOP,
  title={Proof of principle and first cases using preimplantation genetic haplotyping--a paradigm shift for embryo diagnosis.},
  author={Pamela J Renwick and Jane L Trussler and Elham Ostad-Saffari and Hiva Fassihi and Cheryl Black and Peter Braude and Caroline M. Ogilvie and Stephen J. Abbs},
  journal={Reproductive biomedicine online},
  year={2006},
  volume={13 1},
  pages={
          110-9
        }
}
Preimplantation genetic haplotyping (PGH) proof-of-principle was demonstrated by multiple displacement amplification (MDA) of single buccal cells from a female donor and genotyping using 12 polymorphic markers within the dystrophin gene; the known paternal genotype enabled identification of the paternal haplotype in the MDA products despite 27% allele dropout. MDA amplified DNA from 49 single human blastomeres with 100% success. The MDA products were genotyped using a total of 57 polymorphic… CONTINUE READING

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