• Corpus ID: 74368830

Promotion Effect of Black Sesame Oil on Hair Growth in an Alopecia Model of C57BL/6 Mice

@article{Kong2010PromotionEO,
  title={Promotion Effect of Black Sesame Oil on Hair Growth in an Alopecia Model of C57BL/6 Mice},
  author={Min-Kyu Kong and Young-chul Kim},
  journal={Journal of Biomedical Research},
  year={2010},
  volume={11},
  pages={103-116}
}

• Min-Kyu Kong, Young-chul Kim
• Published 30 June 2010
• Biology
• Journal of Biomedical Research

This study was carried out to investigate the effect of black sesame oil on hair growth in a shaving animal model of C57BL/6 mice. Five weeks old male mice were acclimated for one week under 22 ± 1μl room temperature, 50 ± 5% relative humidity and 12 hours of a light/dark cycle before beginning experiment. The animals were divided into 3 groups including normal group (saline, N), positive control group (3% MXD, PC), and experimental group (black sesame oil, E) and they were topically applied with an amount of 100μl once a day, 6 days a week for 3 weeks. Hair regrowth was evaluated by the gross and histological examination. Also immunohistochemical observation for SCF, the activities of enzymes including ALP and γ-GT, and the expression quantity of growth factor including IGF-1 in the skin of mice were carried out or evaluated. Topical treatment of black sesame oil and 3% minoxidil for 3 weeks to dorsal skin accelerated hair regrowth faster than N group. E and PC groups promoted development and elongation of hair follicles compared with N group. Serum ALP activity was significantly higher in E group compared with N group in 3 weeks (p<0.05). Skin ALP activity were significantly higher in PC group compared with N group in 2 weeks (p<0.05), and E and PC groups were higher than N group in 3 weeks with no significant differences. Serum γ-GT activity was significantly higher in PC group compared with N group in 2 weeks (p<0.01), and E group was significantly higher compared with N group in 3 weeks (p<0.05). Skin γ-GT activity was significantly higher in PC group compared with N group in 2 weeks (p<0.05), and PC (p<0.01) and E (p<0.001) groups were significantly higher compared with N group in 3 weeks. IGF-1 expression in the skin was significantly higher in PC and E groups compared with N group (p<0.01). SCF antigens were heavily stained in bulge, stem cell and dermal papilla and middle stained in inner root sheath, outer root sheat and epidermis in E and PC groups. These results indicate that black sesame oil effectively stimulated hair growth in an animal model via several mechanisms and that it can be used practically for hair growth or prevention of hair loss in human beings.