Promoting scopolamine accumulation in transgenic plants of Atropa belladonna generated from hairy roots with over expression of pmt and h6h gene.

@article{Liu2010PromotingSA,
  title={Promoting scopolamine accumulation in transgenic plants of Atropa belladonna generated from hairy roots with over expression of pmt and h6h gene.},
  author={Xiaoqiang Liu and Chunxian Yang and Min Chen and Mingyang Li and Z. Liao and K. Tang},
  journal={Journal of Medicinal Plants Research},
  year={2010},
  volume={4},
  pages={1708-1713}
}
Atropa belladonna is the most important commercial source for obtaining pharmaceutical tropane alkaloids such as scopolamine and hyoscyamine. In the present study, two rate-limiting enzyme genes including putrescine N-methyltransferase and hyoscyamine 6β-hydroxylase were introduced into A. belladonna, and integration of the pmt and h6h genes into the genomic DNA of transgenic plants were confirmed by genomic polymerase chain reaction (PCR) analysis. The scopolamine content of transgenic lines… Expand

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