Promoter sites in the genome of B. subtilis phage SPP1


Transcriptional complexes formed in vitro using DNA of B. subtilis phage SPP1 as template and E. coli and B. subtilis RNA polymerases were analyzed by electron microscopy. Both enzymes recognize the same five strong promoters in the early region of the genome. Strand selection at these sites was identical with both enzymes. These results correlate well with data obtained from in vivo transcription studies. Transcriptional activity in the late region of the genome was very low, not permitting the identification of promoter sites.

DOI: 10.1007/BF00428745

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@article{Stueber1981PromoterSI, title={Promoter sites in the genome of B. subtilis phage SPP1}, author={Dietrich Stueber and Giovanna Morelli and Hermann Bujard and Maria Alejandra Montenegro and Thomas A. Trautner}, journal={Molecular and General Genetics MGG}, year={1981}, volume={181}, pages={518-521} }