Promoter sequence analysis in Bacillus and Escherichia: construction of strong promoters in E. coli.

@article{Yamada1991PromoterSA,
  title={Promoter sequence analysis in Bacillus and Escherichia: construction of strong promoters in E. coli.},
  author={Michiyuki Yamada and Mitsumasa Kubo and Tsuyoshi Miyake and R N Scott Sakaguchi and Yakichi Higo and Tsuneo Imanaka},
  journal={Gene},
  year={1991},
  volume={99 1},
  pages={109-14}
}
Many derivatives of the nprM promoter of Bacillus stearothermophilus and the strong early promoter, A3, of coliphage T3 were designed and chemically synthesized. These promoters consisted of some or all of the AT box, consensus sequence, tac promoter sequence, spacer, and lac operator. The promoter activities were assessed by their ability to express the cat gene in Escherichia coli. One of the derivatives of the A3 promoter, which contained the lac operator, was much stronger (about 3.5 times… CONTINUE READING

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