Promoter selectivity of Escherichia coli RNA polymerase E sigma 70 and E sigma 38 holoenzymes. Effect of DNA supercoiling.

@article{Kusano1996PromoterSO,
  title={Promoter selectivity of Escherichia coli RNA polymerase E sigma 70 and E sigma 38 holoenzymes. Effect of DNA supercoiling.},
  author={Shuichi Kusano and Qingming Ding and Naoyuki Fujita and Akira Ishihama},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 4},
  pages={1998-2004}
}
The functional specificity was compared between two sigma factors, sigma 70 (the major sigma at exponentially growing phase) and sigma 38 (the essential sigma at stationary growth phase), of Escherichia coli RNA polymerase. The core enzyme binding affinity of sigma 38 was less than half the level of sigma 70 as measured by gel filtration column chromatography or by titrating the concentration of sigma required for the maximum transcription in the presence of a fixed amount of core enzyme. In… CONTINUE READING

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