Promoter search by Escherichia coli RNA polymerase on a circular DNA template.

@article{Singer1987PromoterSB,
  title={Promoter search by Escherichia coli RNA polymerase on a circular DNA template.},
  author={P T Singer and Cindy W Wu},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 29},
  pages={14178-89}
}
Using the rapid-mixing/photocross-linking technique developed in our laboratory, we have investigated the kinetics of interaction between Escherichia coli RNA polymerase and pAR1319, a recombinant plasmid DNA containing the bacteriophage T7 A2 early promoter. By monitoring the time-dependent density of bound RNA polymerase along the relaxed circular DNA molecule using this technique, we have been able to demonstrate kinetic evidence for linear diffusion of RNA polymerase along DNA in a… CONTINUE READING

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