Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.

Abstract

The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.

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@article{Katzen1996PromoterAO, title={Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.}, author={F Katzen and Allan Barry Becker and {\'A}ngeles Zorreguieta and Alfred P{\"{u}hler and Luis Ielpi}, journal={Journal of bacteriology}, year={1996}, volume={178 14}, pages={4313-8} }