Prominent mitochondrial DNA recombination intermediates in human heart muscle.

@article{Kajander2001ProminentMD,
  title={Prominent mitochondrial DNA recombination intermediates in human heart muscle.},
  author={Olli A. Kajander and Pekka J. Karhunen and Ian Holt and Howard T. Jacobs},
  journal={EMBO reports},
  year={2001},
  volume={2 11},
  pages={1007-12}
}
Recombination intermediates containing four-way (Holliday) junctions are generated during DNA repair and replication in many systems, including yeast mitochondrial DNA (mtDNA). In contrast, convincing evidence for recombination in mammalian mtDNA is lacking. We have used two-dimensional agarose-gel electrophoresis to analyse non-linear forms of mtDNA in human heart muscle. Replication intermediates from both the coupled and strand-asynchronous mtDNA replication pathways were detected. An… CONTINUE READING