Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity.

  title={Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity.},
  author={Mickel Leonardus August Jansen and Jasper A. Diderich and Mlawule R. Mashego and Adham Hassane and Johannes H. de Winde and Pascale A. S. Daran-Lapujade and Jack T. Pronk},
  volume={151 Pt 5},
Prolonged cultivation of Saccharomyces cerevisiae in aerobic, glucose-limited chemostat cultures (dilution rate, 0.10 h(-1)) resulted in a progressive decrease of the residual glucose concentration (from 20 to 8 mg l(-1) after 200 generations). This increase in the affinity for glucose was accompanied by a fivefold decrease of fermentative capacity, and changes in cellular morphology. These phenotypic changes were retained when single-cell isolates from prolonged cultures were used to inoculate… 

Figures and Tables from this paper

Control of the Glycolytic Flux in Saccharomyces cerevisiae Grown at Low Temperature

To investigate how yeast maintained a constant glycolytic flux despite the kinetic challenge imposed by a lower growth temperature, a systems approach was applied that involved metabolic flux analysis, transcript analysis, enzyme activity assays, and metabolite analysis.

Dynamics of Glycolytic Regulation during Adaptation of Saccharomyces cerevisiae to Fermentative Metabolism

This multilevel regulation study qualitatively explains the increase in flux through the glycolytic enzymes upon a switch of S. cerevisiae to fermentative conditions and provides a better understanding of the roles of different regulatory mechanisms that influence the dynamics of yeast Glycolysis.

Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant Saccharomyces cerevisiae under Glucose-Limited Conditions

It is shown that three apparent phenotypes underlie the adaptive response observed at the bulk level in the chemostat, which highlights the importance of considering population heterogeneity when studying adaptation in industrial bioprocesses.

Acclimation of Saccharomyces cerevisiae to low temperature: a chemostat-based transcriptome analysis.

Comparison of the chemostat-based transcriptome data with literature data revealed large differences between transcriptional reprogramming during long-term low-temperature acclimation and the transcriptional responses to a rapid transition to low temperature.

Slow Adaptive Response of Budding Yeast Cells to Stable Conditions of Continuous Culture Can Occur without Genome Modifications

It is shown that in a population of budding yeast cells grown for over 200 generations in continuous culture in non-limiting minimal medium and therefore not subject to selection pressure, remodeling of transcriptome occurs, but not as a result of the accumulation of adaptive mutations.

Fluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of Saccharomyces cerevisiae

It is found that the transition can be completely avoided in the presence of fluctuations in glucose availability as the strains subjected to feast–famine conditions under otherwise constant culture conditions exhibited constant levels of the measured proteome for over 250 hr.

Large excess capacity of glycolytic enzymes in Saccharomyces cerevisiae under glucose-limited conditions

Computational modeling is used to suggest that in yeast the overcapacity of the glycolytic enzymes at low specific growth rates is a genuine excess, rather than the optimal enzyme demand dictated by enzyme kinetics, and it is argued that the excess capacity of glyCOlytic proteins in glucose-scarce conditions is an adaptation of S. cerevisiae to fluctuations of nutrient availability in the environment.

Anaerobic homolactate fermentation with Saccharomyces cerevisiae results in depletion of ATP and impaired metabolic activity.

The anticipated increase in maximal velocity (V(max) of glycolytic enzymes was not observed in homolactate fermentation suggesting the absence of protein synthesis that may be attributed to decreased energy availability, which hinders protein synthesis, central carbon metabolism and subsequent energy generation.

Effect of Nutrient Starvation on the Cellular Composition and Metabolic Capacity of Saccharomyces cerevisiae

The results showed that the glucose transport capacity is a key factor for maintenance of high metabolic capacity in many, but not all, cases.

An excess of glycolytic enzymes under glucose‐limited conditions may enable Saccharomyces cerevisiae to adapt to nutrient availability

Computational modelling is used to suggest that yeast maintains a genuine excess of glycolytic enzymes at low specific growth rates, which is a preparatory strategy in the adaptation to sugar fluctuations in the environment.



Use of a glycerol-limited, long-term chemostat for isolation of Escherichia coli mutants with improved physiological properties.

Two mutants were isolated that exhibited generally improved growth phenotypes in batch cultivations on glycerol, glucose or the gluconeogenic substrate acetate and exhibited increased resistance to a variety of adverse conditions including heat shock, osmotic stress and nutrient deprivation.

The Role Of Limited Respiration In The Incomplete Oxidation Of Glucose By Saccharomyces Cerevisiae

A limitation in some step in the oxidative branch of catabolism is likely to be responsible for incomplete oxidation of glucose at high growth rates rather than an undefined action of glucose repression.

Selection of glucose-assimilating variants ofAcinetobacter calcoaceticus LMD 79.41 in chemostat culture

In contrast to the starter culture, cells from chemostats which had been fully adapted to gluconate utilization, were able to utilize glucose as a sole carbon and energy source in liquid and solid media.

Physiological characterization of adaptive clones in evolving populations of the yeast, Saccharomyces cerevisiae.

Populations of a diploid strain of S. cerevisiae were grown in glucose-limited continuous culture for more than 260 generations. A series of seven sequential adaptive changes were identified by

Regulation of carbon metabolism in chemostat cultures of Saccharomyces cerevisiae grown on mixtures of glucose and ethanol

It is concluded that, during carbon‐limited growth of S. cerevisiae on mixtures of glucose and ethanol, biosynthetic intermediates with three or more carbon atoms are preferentially synthesized from glucose.

Role of Transcriptional Regulation in Controlling Fluxes in Central Carbon Metabolism of Saccharomyces cerevisiae

Results indicate that in vivo fluxes in the central carbon metabolism of S. cerevisiae grown in steadystate, carbon-limited chemostat cultures are controlled to a large extent via post-transcriptional mechanisms.

Multiple duplications of yeast hexose transport genes in response to selection in a glucose-limited environment.

A population of baker's yeast that underwent 450 generations of glucose-limited growth is analyzed and the existence of multiple tandem duplications involving two highly similar, high-affinity hexose transport loci, HXT6 and HXT7, is revealed.