Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity.

@article{Jansen2005ProlongedSI,
  title={Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity.},
  author={Mickel Leonardus August Jansen and Jasper A. Diderich and Mlawule R. Mashego and Adham Hassane and Johannes H. de Winde and Pascale A. S. Daran-Lapujade and Jack T. Pronk},
  journal={Microbiology},
  year={2005},
  volume={151 Pt 5},
  pages={
          1657-69
        }
}
Prolonged cultivation of Saccharomyces cerevisiae in aerobic, glucose-limited chemostat cultures (dilution rate, 0.10 h(-1)) resulted in a progressive decrease of the residual glucose concentration (from 20 to 8 mg l(-1) after 200 generations). This increase in the affinity for glucose was accompanied by a fivefold decrease of fermentative capacity, and changes in cellular morphology. These phenotypic changes were retained when single-cell isolates from prolonged cultures were used to inoculate… 

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References

SHOWING 1-10 OF 67 REFERENCES

Use of a glycerol-limited, long-term chemostat for isolation of Escherichia coli mutants with improved physiological properties.

Two mutants were isolated that exhibited generally improved growth phenotypes in batch cultivations on glycerol, glucose or the gluconeogenic substrate acetate and exhibited increased resistance to a variety of adverse conditions including heat shock, osmotic stress and nutrient deprivation.

The Role Of Limited Respiration In The Incomplete Oxidation Of Glucose By Saccharomyces Cerevisiae

A limitation in some step in the oxidative branch of catabolism is likely to be responsible for incomplete oxidation of glucose at high growth rates rather than an undefined action of glucose repression.

Selection of glucose-assimilating variants ofAcinetobacter calcoaceticus LMD 79.41 in chemostat culture

In contrast to the starter culture, cells from chemostats which had been fully adapted to gluconate utilization, were able to utilize glucose as a sole carbon and energy source in liquid and solid media.

Physiological characterization of adaptive clones in evolving populations of the yeast, Saccharomyces cerevisiae.

Populations of a diploid strain of S. cerevisiae were grown in glucose-limited continuous culture for more than 260 generations. A series of seven sequential adaptive changes were identified by

Regulation of carbon metabolism in chemostat cultures of Saccharomyces cerevisiae grown on mixtures of glucose and ethanol

It is concluded that, during carbon‐limited growth of S. cerevisiae on mixtures of glucose and ethanol, biosynthetic intermediates with three or more carbon atoms are preferentially synthesized from glucose.

Role of Transcriptional Regulation in Controlling Fluxes in Central Carbon Metabolism of Saccharomyces cerevisiae

Results indicate that in vivo fluxes in the central carbon metabolism of S. cerevisiae grown in steadystate, carbon-limited chemostat cultures are controlled to a large extent via post-transcriptional mechanisms.

Multiple duplications of yeast hexose transport genes in response to selection in a glucose-limited environment.

A population of baker's yeast that underwent 450 generations of glucose-limited growth is analyzed and the existence of multiple tandem duplications involving two highly similar, high-affinity hexose transport loci, HXT6 and HXT7, is revealed.
...