Prolonged culture of telomerase-immortalized human fibroblasts leads to a premalignant phenotype.

@article{Milyavsky2003ProlongedCO,
  title={Prolonged culture of telomerase-immortalized human fibroblasts leads to a premalignant phenotype.},
  author={Michael Milyavsky and Igor Shats and Neta Erez and Xiaohu Tang and Shai Senderovich and Ari Meerson and Yuval Tabach and Naomi Goldfinger and Doron Ginsberg and Curtis C. Harris and Varda Rotter},
  journal={Cancer research},
  year={2003},
  volume={63 21},
  pages={
          7147-57
        }
}
Telomere shortening in primary human fibroblasts results in replicative senescence, which can be overcome by telomerase (hTERT) overexpression. However, because immortalization is one of the hallmarks of malignant transformation, careful analysis of hTERT-immortalized cells is of crucial importance for understanding both processes. To this end, we infected WI-38 fibroblasts with a retrovirus carrying the hTERT cDNA and analyzed their proliferative behavior during 600 days [ approximately 500… CONTINUE READING

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Use of genetic suppressor elements to dissect distinct biological effects of separate p53 domains.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 1996
VIEW 8 EXCERPTS
HIGHLY INFLUENTIAL