Prolonged cryopreservation of human bone marrow.

  title={Prolonged cryopreservation of human bone marrow.},
  author={Leroy Monroe Parker and Nicole Binder and Rebecca S. Gelman and Carol M. Richman and Roy S Weiner and Ronald A. Yankee},
  volume={31 6},
We tested the viability of human bone marrow stored for 40 to 42 months in the vapor phase of liquid nitrogen. A median of 2 X 10(10) nucleated cells obtained from eight patients were concentrated to 1.3 X 10(10) using discontinuous centrifugation. These were stored in polyolefin bags in volumes of 100 to 500 ml using 10% dimethyl sulfoxide (DMSO) as… CONTINUE READING