Proline isomerization during refolding of ribonuclease A is accelerated by the presence of folding intermediates.

@article{Schmid1986ProlineID,
  title={Proline isomerization during refolding of ribonuclease A is accelerated by the presence of folding intermediates.},
  author={Franz Xaver Schmid},
  journal={FEBS letters},
  year={1986},
  volume={198 2},
  pages={217-20}
}
The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had been converted to a folding intermediate by a 15 s refolding pulse under strongly native conditions (0.8 M ammonium sulfate, 0 degree C). The results demonstrate that extensive… CONTINUE READING