Proline is required for the stimulation of DNA synthesis in hepatocyte cultures by EGF

@article{Houck2007ProlineIR,
  title={Proline is required for the stimulation of DNA synthesis in hepatocyte cultures by EGF},
  author={Keith A. Houck and George K. Michalopoulos},
  journal={In Vitro Cellular \& Developmental Biology},
  year={2007},
  volume={21},
  pages={121-124}
}
SummaryEpidermal growth factor (EGF) has been shown to stimulate DNA synthesis in rat parenchymal hepatocytes both in vivo and in vitro (4,9). We report here that this response in vitro is dependent on the amino acids present in the media. Of all the amino acids, proline has the strongest effect. The response to EGF is absent without proline and none of the other amino acids can substitute for it. Added proline (1 mM) to the media caused the labeling index to increase from 11% to 55% in the… 
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15 Citations
Background Citations
7
Results Citations
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15 Citations

The bicarbonate ion is essential for efficient DNA synthesis by primary cultured rat hepatocytes
TLDR
A dose response of NaHCO3 in several media showed that DNA synthesis of the cells increased as the concentration of NahCO3 increased and that 25 to 30 mM NaH CO3 in the medium was optimal for the replication of DNA by primary cultured rat hepatocytes.
Effects of extracellular matrix components on the growth and differentiation of cultured rat hepatocytes
TLDR
It is demonstrated that specific components of the extracellular matrix modulate both differentiated functions and the replication of hepatocytes cultured in serum-free medium.
Hepatopoietins A and B and hepatocyte growth
TLDR
The finding that serum from control or partially hepatectomized rats contains only two substances associated with stimulation of DNA synthesis in primary cultures of hepatocytes in serum free conditions raises the issue of the overall importance of different heparin binding growth factors in the control of hepatic growth regulation.
Mechanisms controlling growth of hepatocytes in primary culture
  • A. Ichihara
  • Biology, Medicine
    Digestive Diseases and Sciences
  • 2005
TLDR
The mechanisms of liver regeneration, differentiation, and hepatocarcinogenesis are discussed and a hepatocyte growth factor that is isolated from rat platelets is identified.
Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolated at Different Times after Partial Hepatectomy1
TLDR
Primary hepatocyte cultures have been prepared from normal adult rat liver and from rat liver at 4, 8,12,24, and 48 h following partial hepatectomy and addition of epidermal growth factor alone or in combination with insulin resulted in a dramatic increase in DNA synthesis in hepatocytes from regenerating rat liver.
Up-regulation of cyclin-E(1) via proline-mTOR pathway is responsible for HGF-mediated G(1)/S progression in the primary culture of rat hepatocytes.
A simple medium for the study of hepatocyte growth in culture under defined conditions
SummaryThe combination (1∶1) of Dulbecco's modified Eagle's medium and Waymouth's medium MAB 87/3 was found to provide favorable conditions for serum-free culture and growth of adult rat hepatocytes.…
Transforming growth factor-β 1 stimulates vascular smooth muscle cell L-proline transport by inducing system A amino acid transporter 2 ( SAT 2 ) gene expression
TLDR
Kinetic studies indicated that TGF-β1-induced -proline transport was mediated by an increase in transport capacity independent of any changes in the affinity for �‬- Proline.
Synthesis of a Biologically Active Tethered Growth Factor Surface and Comparison with Soluble Delivery
TLDR
In order to generalize the tethering method for polymer substrates, and to provide more control of ligand density and spacing on the surface, EGF was covalently coupled to star PEO along with a bifunctional crosslinking molecule, ethylene diamine.
The development of a novel in vitro model of human liver for the study of disease pathogenesis
TLDR
This project compares the established 3D model, co-culture of hepatocytes and hepatic stellate cells (HSCs) on PDLLA coated surfaces, to other best available systems using collagen and Matrigel to demonstrate the hypothesis that hepatocyte functions of the established model can be further improved by introduction of cell-matrix interaction.
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TLDR
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The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in…
Purification of proline-rich proteins from parotid glands of isoproterenol-treated rats.
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