Proline is required for the stimulation of DNA synthesis in hepatocyte cultures by EGF

  title={Proline is required for the stimulation of DNA synthesis in hepatocyte cultures by EGF},
  author={Keith A. Houck and George K. Michalopoulos},
  journal={In Vitro Cellular \& Developmental Biology},
SummaryEpidermal growth factor (EGF) has been shown to stimulate DNA synthesis in rat parenchymal hepatocytes both in vivo and in vitro (4,9). We report here that this response in vitro is dependent on the amino acids present in the media. Of all the amino acids, proline has the strongest effect. The response to EGF is absent without proline and none of the other amino acids can substitute for it. Added proline (1 mM) to the media caused the labeling index to increase from 11% to 55% in the… 
The bicarbonate ion is essential for efficient DNA synthesis by primary cultured rat hepatocytes
A dose response of NaHCO3 in several media showed that DNA synthesis of the cells increased as the concentration of NahCO3 increased and that 25 to 30 mM NaH CO3 in the medium was optimal for the replication of DNA by primary cultured rat hepatocytes.
Effects of extracellular matrix components on the growth and differentiation of cultured rat hepatocytes
It is demonstrated that specific components of the extracellular matrix modulate both differentiated functions and the replication of hepatocytes cultured in serum-free medium.
Hepatopoietins A and B and hepatocyte growth
The finding that serum from control or partially hepatectomized rats contains only two substances associated with stimulation of DNA synthesis in primary cultures of hepatocytes in serum free conditions raises the issue of the overall importance of different heparin binding growth factors in the control of hepatic growth regulation.
Mechanisms controlling growth of hepatocytes in primary culture
  • A. Ichihara
  • Biology, Medicine
    Digestive Diseases and Sciences
  • 2005
The mechanisms of liver regeneration, differentiation, and hepatocarcinogenesis are discussed and a hepatocyte growth factor that is isolated from rat platelets is identified.
Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolated at Different Times after Partial Hepatectomy1
Primary hepatocyte cultures have been prepared from normal adult rat liver and from rat liver at 4, 8,12,24, and 48 h following partial hepatectomy and addition of epidermal growth factor alone or in combination with insulin resulted in a dramatic increase in DNA synthesis in hepatocytes from regenerating rat liver.
A simple medium for the study of hepatocyte growth in culture under defined conditions
SummaryThe combination (1∶1) of Dulbecco's modified Eagle's medium and Waymouth's medium MAB 87/3 was found to provide favorable conditions for serum-free culture and growth of adult rat hepatocytes.
Transforming growth factor-β 1 stimulates vascular smooth muscle cell L-proline transport by inducing system A amino acid transporter 2 ( SAT 2 ) gene expression
Kinetic studies indicated that TGF-β1-induced -proline transport was mediated by an increase in transport capacity independent of any changes in the affinity for �‬- Proline.
Synthesis of a Biologically Active Tethered Growth Factor Surface and Comparison with Soluble Delivery
In order to generalize the tethering method for polymer substrates, and to provide more control of ligand density and spacing on the surface, EGF was covalently coupled to star PEO along with a bifunctional crosslinking molecule, ethylene diamine.
The development of a novel in vitro model of human liver for the study of disease pathogenesis
This project compares the established 3D model, co-culture of hepatocytes and hepatic stellate cells (HSCs) on PDLLA coated surfaces, to other best available systems using collagen and Matrigel to demonstrate the hypothesis that hepatocyte functions of the established model can be further improved by introduction of cell-matrix interaction.


Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes.
A prominent role for epidermal growth factor is suggested in promoting hepatic DNA synthesis by acting in concert with insulin and glucagon in cultured hepatocytes from partially hepatectomized rats.
DNA synthesis in primary cultures of adult rat hepatocytes in a defined medium: Effects of epidermal growth factor, insulin, glucagon, and cyclic‐AMP
In primary monolayer cultures of freshly isolated adult rat liver parenchymal cells, in which contamination with nonparental cells was negligible, DNA synthesis was substantially stimulated by these substances, including EGF, glucagon, or cyclic‐AMP.
Rapid degradation of newly synthesized collagen by primary cultures of adult rat hepatocytes.
Unscheduled DNA synthesis induced by procarcinogens in suspensions and primary cultures of hepatocytes on collagen membranes.
The results demonstrate that hepatocytes cultured on collagen membranes can metabolize chemical carcinogens and the selective advantages of the two systems of hepatocytes can be utilized for the establishment of short-term in vitro screening systems of mutagens and carcinogens.
Liver regeneration studies with rat hepatocytes in primary culture.
The results show that this system of replicating hepatocytes can be used to investigate the trophic factors that control growth of normal and neoplastic hepatocytes.
Amino acid metabolism in mammalian cell cultures.
The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in
Purification of proline-rich proteins from parotid glands of isoproterenol-treated rats.
Types of Collagen Synthesized by Normal Rat Liver Hepatocytes in Primary Culture
Collagen formation is an important function of liver parenchymal cells that may be relevant to the pathogenesis of hepatic fibrosis and is identified by ion exchange chromatography and by polyacrylamide gel electrophoresis.
Primary cultures of hepatocytes on human fibroblasts
The similarities in performance between hepatocytes on collagen membranes and on human fibroblasts show that a continuous collagen-containing substratum is important for optimal performance of hepatocytes in primary culture.
Transplantation system for determining the clonogenic survival of parenchymal hepatocytes exposed to ionizing radiation.
Results indicate that hepatocytes irradiated while in the G0 phase are unable to accumulate sublethal damage to an appreciable extent if they are stimulated to undergo replication within 24 hr after the infliction of the damage.