Proline is required for the stimulation of DNA synthesis in hepatocyte cultures by EGF

@article{Houck2007ProlineIR,
  title={Proline is required for the stimulation of DNA synthesis in hepatocyte cultures by EGF},
  author={Keith A. Houck and George K. Michalopoulos},
  journal={In Vitro Cellular \& Developmental Biology},
  year={2007},
  volume={21},
  pages={121-124}
}
SummaryEpidermal growth factor (EGF) has been shown to stimulate DNA synthesis in rat parenchymal hepatocytes both in vivo and in vitro (4,9). We report here that this response in vitro is dependent on the amino acids present in the media. Of all the amino acids, proline has the strongest effect. The response to EGF is absent without proline and none of the other amino acids can substitute for it. Added proline (1 mM) to the media caused the labeling index to increase from 11% to 55% in the… 
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Transforming growth factor-β 1 stimulates vascular smooth muscle cell L-proline transport by inducing system A amino acid transporter 2 ( SAT 2 ) gene expression
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Kinetic studies indicated that TGF-β1-induced -proline transport was mediated by an increase in transport capacity independent of any changes in the affinity for �‬- Proline.
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This project compares the established 3D model, co-culture of hepatocytes and hepatic stellate cells (HSCs) on PDLLA coated surfaces, to other best available systems using collagen and Matrigel to demonstrate the hypothesis that hepatocyte functions of the established model can be further improved by introduction of cell-matrix interaction.
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