Proline Accumulation, Protein Pattern and Photosynthesis in Bacopa Monniera Regenerants Grown under NaCl Stress

  title={Proline Accumulation, Protein Pattern and Photosynthesis in Bacopa Monniera Regenerants Grown under NaCl Stress},
  author={Gayoor Ali and Prem Shankar Srivastava and Muhammad Asif Iqbal},
  journal={Biologia Plantarum},
Shoots of Bacopa monniera exhibited 100 % regeneration on Murashige and Skoog medium with 2 % sucrose, 0.2 mg dm−3 1-naphthaleneacetic acid, 0.5 mg dm−3 6-benzylaminopurine and 50 mg dm−3 glutamine. When the medium was supplied with various concentrations (5 - 15 g dm−3) of sodium chloride, proline content in regenerants was six times higher than in the control. With increasing NaCl concentration photosynthetic rate decreased and fresh mass and root length of regenerants declined. NaCl also… 

Growth and Metabolism of Senna as Affected by Salt Stress

Pot culture experiments were conducted using different NaCl concentrations to assess their impact on the growth and metabolic changes in senna (Cassia angustifolia Vahl.). Five treatments (0, 40, 80,

Structural changes in root and shoot ofBacopa monniera in response to salt stress

Various concentrations of salt (NaCI) were shown to have an influence on the differentiation of tissues in the root and stem ofBacopa monniera (L) Wettst. Higher concentrations induced drastic

Influence of Cadmium and Zinc on Growth and Photosynthesis of Bacopa monniera Cultivated in vitro

This study evaluates the impact of cadmium and zinc interaction on the amount of soluble proteins, CO2 fixati stomatal conductance and intercellular CO2 contents in regenerants of B. monniera. The

Effect of salt stress on growth and biochemical parameters of Pisum sativum L.

High NaCl concentrations caused a great reduction in growth parameters such as fresh and dry weight of leaves and roots, but the leaf number was less affected, and the proline and sugar content was increased, but nitrate reductase activity and chlorophyll was found to decrease in leaves.

Effect of sodium chloride-induced stress on growth, proline, glycine betaine accumulation, antioxidative defence and bacoside A content in in vitro regenerated shoots of Bacopa monnieri (L.) Pennell

Growth, osmotic adjustment, antioxidant enzyme defense and the principle medicinal component bacoside A were studied in the in vitro raised shoot cultures of Bacopa monnieri, a known medicinal plant,

Response of two mustard (Brassica juncea L.) cultivars differing in photosynthetic capacity subjected to proline

Exogenous application of proline significantly increased plant growth, photosynthetic rate, and the activities of antioxidant enzymes, compared with untreated seedlings.

Effect of cadmium and copper on growth of Bacopa monniera regenerants

The effects of cadmium and copper on in vitro growth of Bacopa monniera (L.) Wettst. was monitored. Cadmium (25 and 50 µM CdCl2) inhibited plantlet growth and addition of 50 or 100 µm CuSO4 partially

Ameliorative Effects of CaCl2 on Growth, Ionic Relations, and Proline Content of Senna Under Salinity Stress

ABSTRACT The effect of NaCl and CaCl2 on growth parameters, ionic relations, and proline level in senna (Cassia angustifolia) plant was investigated in a pot culture experiment using NaCl (80 mM and

Superoxide dismutase isozyme activity and antioxidant responses of hydroponically cultured Lepidium sativum L. to NaCl stress

Findings proved that L. sativum can be classified as a moderately tolerant plant to salinity and demonstrated that activities of antioxidant defense enzymes changed in parallel with the increased salinity.

Effect of potassium chloride and calcium chloride induced stress on in vitro cultures of Bacopa monnieri (L.) Pennell and accumulation of medicinally important bacoside A

It appears from the data that accumulation of osmolytes, and elevated activities of antioxidant enzymes play an important role in osmotic adjustment in shoot cultures of Bacopa and the two salts tested have a positive effect on bacoside accumulation.



The role of proline accumulation in halophytes

In the majority of higher plant halophytes examined proline is the major component of the amino acid pool in plants collected from the field and it is suggested that proline functions as a source of solute for intracellular osmotic adjustments under saline conditions.

Lipid and protein changes in jojoba callus under salt stress

Polypeptides of 18, 27 and 49 kDa, which accumulated in salt-treated calluses appeared as marker proteins for salt adaptation in jojoba calluses.

Effect of Salt Stress on Callus Cultures of Oryza sativa L.

Kavi Kishor, P. B. 1988. Effect of salt stress on callus cultures of Oryza sativa L.—J. exp. Bot. 39: 235-240. Callus cultures of rice adapted to grow under increasing NaCl stress were found to

Salt stress in cultured rice cells: effects of proline and abscisic acid

Abscisic acid (ABA) supressed the growth of both salt-adapted and unadapted callus of rice in the absence of salt stress and increased the growth among cells exposed to salt but not to water deficits imposed by nonionic solutes.

Protein synthesis in a maize callus exposed to NaCl and mannitol

Severe inhibition of uptake and protein synthesis results from prolonged exposures at growth-inhibitory concentrations of NaCl and mannitol, and altered expression of newly-made proteins in the maize callus occurs only after a week of exposure to salinity or osmotic stress and coincides with the cell growth phase.

Abscisic Acid accelerates adaptation of cultured tobacco cells to salt.

The results suggest that ABA is involved in adaptation of cells to salts, and is not effective in promoting adaptation to water deficits elicited by nonionic osmotic solutes.