Prolific direct plant regeneration from cotyledons of white clover

@article{White1994ProlificDP,
  title={Prolific direct plant regeneration from cotyledons of white clover},
  author={Derek William Richard White and Christine R. Voisey},
  journal={Plant Cell Reports},
  year={1994},
  volume={13},
  pages={303-308}
}
A facile procedure has been developed to regenerate white clover (Trifolium repens L.) plants, rapidly and directly from cotyledon explants of 3 day old seedlings. Scanning electron microscopy and histological sectioning demonstrated that shoot meristems developed from individual epidermal cells on the adaxial surface of the cotyledonary stalk, proximal to the site of excision. Initial cell divisions occurred after 2 days of culture and regenerated plants were transferred to soil within 6–8… CONTINUE READING
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