Proliferation of epidermal growth factor-stimulated hepatocytes in a hormonally defined serum-free medium.

@article{Papeleu2004ProliferationOE,
  title={Proliferation of epidermal growth factor-stimulated hepatocytes in a hormonally defined serum-free medium.},
  author={Peggy Papeleu and Pascal Loyer and Tamara Vanhaecke and Tom Henkens and Greetje Elaut and Christiane Guguen-Guillouzo and Vera Maria Rogiers},
  journal={Alternatives to laboratory animals : ATLA},
  year={2004},
  volume={32 Suppl 1A},
  pages={
          57-64
        }
}
The present study shows that adult rat hepatocytes in primary culture, which normally exhibit a restricted capacity to proliferate, can proceed through the cell cycle when cultured in a mixture of minimal essential medium (MEM) and Medium 199 (MEM-M199; 3:1, v/v), containing epidermal growth factor (EGF; 50 ng/ml), low glucose (0.75 g/l) and low levels of inorganic salts, amino acids and vitamins. Under these conditions, hepatocytes flatten and cell extensions appear. In contrast, Dulbecco's… CONTINUE READING