Proliferation, differentiation, and long-term culture of primary hippocampal neurons.

  title={Proliferation, differentiation, and long-term culture of primary hippocampal neurons.},
  author={Jasodhara Ray and Daniel A. Peterson and M Schinstine and Fred H. Gage},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={90 8},
Primary embryonic hippocampal neurons can develop morphologically and functionally in culture but do not survive more than a few weeks. It has been reported that basic fibroblast growth factor (bFGF) promotes the survival of and neurite elongation from fetal hippocampal neurons. We report that bFGF, in a dose-dependent manner, can induce the survival (50 pg to 1 ng/ml) and proliferation (10-20 ng/ml) of embryonic hippocampal progenitor neurons in vitro. In serum-free medium containing high… CONTINUE READING
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