Prolidase, a potential enzyme target for melanoma: design of proline-containing dipeptide-like prodrugs.

@article{Mittal2005ProlidaseAP,
  title={Prolidase, a potential enzyme target for melanoma: design of proline-containing dipeptide-like prodrugs.},
  author={Sachin Mittal and Xueqin Song and Balvinder Singh Vig and Christopher P. Landowski and Insook Kim and John M. Hilfinger and Gordon L Amidon},
  journal={Molecular pharmaceutics},
  year={2005},
  volume={2 1},
  pages={37-46}
}
Bioinformatics tools such as Perl, Visual Basic, Cluster, and TreeView were used to analyze public gene expression databases in order to identify potential enzyme targets for prodrug strategies. The analyses indicated that prolidase might be a desirable enzyme target based on its differential expression in melanoma cancer cell lines and its high substrate specificity for dipeptides containing proline at the carboxy terminus. RT-PCR expression of prolidase and hydrolytic activity against N… CONTINUE READING