Prolactin receptor mRNA localization in the hypothalamus by in situ hybridization.

@article{Chiu1994ProlactinRM,
  title={Prolactin receptor mRNA localization in the hypothalamus by in situ hybridization.},
  author={Shing Yan Chiu and Phyllis M. Wise},
  journal={Journal of neuroendocrinology},
  year={1994},
  volume={6 2},
  pages={191-9}
}
Prolactin receptors may mediate the action of prolactin in the brain to influence behavior and neuroendocrine secretions. We recently demonstrated prolactin receptor gene expression in the anterior and medial basal hypothalamus and not in the cortex by the reverse transcription-polymerase chain reaction. In this paper, we localize the prolactin receptor gene expression to individual cells with in situ hybridization. Several steps in the in situ hybridization method were modified to increase… CONTINUE READING

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