Prokaryotic gene fusion expression systems and their use in structural and functional studies of proteins.

@article{Sheibani1999ProkaryoticGF,
  title={Prokaryotic gene fusion expression systems and their use in structural and functional studies of proteins.},
  author={Nader Sheibani},
  journal={Preparative biochemistry & biotechnology},
  year={1999},
  volume={29 1},
  pages={77-90}
}
  • Nader Sheibani
  • Published 1999 in Preparative biochemistry & biotechnology
The ability to express and purify large quantity of proteins in bacteria has greatly impacted many aspects of biological research. These include their use as a source of reagent for biochemical and biophysical studies as well as a source of antigen for antibody production. Currently many different expression systems are available and new ones are being developed. These systems allow inducible expression of a desired protein as a fusion with an affinity tag for simple purification. The affinity… CONTINUE READING

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