Prokaryotic functional diversity in different biogeochemical depth zones in tidal sediments of the Severn Estuary, UK, revealed by stable-isotope probing.


Stable isotope probing of prokaryotic DNA was used to determine active prokaryotes using (13)C-labelled substrates (glucose, acetate, CO(2)) in sediment slurries from different biogeochemical zones of the Severn Estuary, UK. Multiple, low concentrations (5 x 100 microM) of (13)C-substrate additions and short-term incubations (7 days) were used to minimize… (More)
DOI: 10.1111/j.1574-6941.2010.00848.x


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