• Biology
  • Published 2012

Prokaryotic Expression of DEV Extracellular gD Gene and Its Subcellular Localization in Virus-infected DEF

@inproceedings{Junwei2012ProkaryoticEO,
  title={Prokaryotic Expression of DEV Extracellular gD Gene and Its Subcellular Localization in Virus-infected DEF},
  author={Wang Jun-wei},
  year={2012}
}
The extracellular gD gene was amplified by PCR from DEV genomic DNA according to our laboratory discovered gene sequence(GenBank accession number EU714267).The recombinant expression plasmid pET-30a-gDw could effectively express gDw fusion protein that was used to immunize rabbits.The titer of antibody tested by agar diffusion reaction was 1:16.Plaque reduction neutralization test showed that its neutralization antibody titer was up to 1:64.By indirected immunofluorescence,the results showed… CONTINUE READING